Protein Expression Facility

The Texas Institute for Drug and Diagnostics Development is currently bringing on-line a Protein Expression Facility.

Expression Equipment

New Brunswick Scientific BioFlo 310 Benchtop Autoclavable Fermentor & Bioreactor System 

The BioFlo 310 benchtop Bioreactor can be used for Batch, fed-batch and continuous fermentation for growing a wide variety of cell types: microbes, yeast and fungi, as well as mammalian, insect and plant cell lines with the use of optional accessories. Interchangeable, autoclavable glass vessels are available in two sizes 3.5 and 14.0 liters (total volume).

BioFlo 310  

New Brunswick Scientific INNOVA 44 Stackable Incubator Shakers 

The Intuitive Programming allows multiple parameter changes-temperature, speed, photosynthetic lights and UV light decontamination. All are easily changed on a timed basis. Ideal for heat inductions or temperature reductions to slow growth, eliminating unnecessary trips back to the lab. Superior Air Circulation System with low-watt-density resistance heaters for rapid temperature equilibration and excellent uniformity. Precisely-Regulated Temperature, ranging from +5ºC above ambient to 80ºC; accurate within ±0.1ºC. Dynamic Speed Range from 25 to 400 rpm controlled within ±1 rpm. Timed Shaking from 0.1 to 99.9 hours, or continuous shaking. Extra-Large Chamber holds flasks up to 5 liters.

INNOVA 44 Stackable Incubator Shakers

BD FACSAria Cell Sorting System 

This is the first benchtop high-speed sorter with fixed-alignment cuvette flow cell capabilities. Cuvette flow cell for superior fluorescence sensitivity, up to three air-cooled lasers at 488-nm, 633-nm, and 407-nm wavelengths. Digital acquisition rates of up to 70,000 events/second. Multicolor analysis of up to 15 parameters, Two- and four-way bulk sorting devices for a variety of tube sizes. Optional BD™ Automated Cell Deposition Unit (ACDU) for sorting to BD Multiwell™ plates or microscope slides.

Cell Sorting System

Biacore Life Sciences - BIAcore 3000 

The BIAcore 3000 system is an instrument that uses SPR technology for measuring the interactions of macromolecules with each other, and with small ligands. One of the ligands is immobilized on carboxymethylated dextran over a gold surface, while the second partner (analyte) is captured as it flows over the immobilized ligand surface. Typical amounts of a protein ligand needed for an immobilization reaction is about 1 µg. The immobilized ligands are remarkably resilient and maintain their biological activity. The bound analytes can be stripped from the immobilized ligand without affecting its activity to allow many cycles of binding and regeneration on the same immobilized surface. Interaction is detected via SPR, in real time, at high sensitivity, without the use of radioactivity. Affinity is measurable in the range of 105 - 5x1011 M-1. Concentration determination experiments are also feasible.

BIAcore 3000

Thermo Electron Corporation - FRENCH Press 

This French press can be used for following applications; Enzyme preparation and isolation, Cell extract preparation, Bacterial and plant tissue disintegration, Recovery of intercellular proteins and nucleic acid, Preparation of viral antigens for EIA test kits, Membrane preparation for rhizobia and pseudomonads, Disruption of bacillus spores and vegetative cells for pigment analysis, and Analytical fractionation of epithelial cells. Two models of FRENCH Pressure Cells are available - The 40K cell has a maximum working pressure of 40,000 psi and a cell capacity of 35ml. The miniature cell for work with small sample volumes holds from 1.4 to 3.7 mL and has a maximum working pressure of 20,000 psi. The FRENCH Pressure Cell delivers higher pressures than bombs or decompression systems, without pre-treating the sample.

French Press

Amersham Akta FPLC 

ÄKTAFPLC™ is a biocompatible, high performance liquid chromatography system for fast and easy purification of proteins. The unique design provides a compact, ready to use chromatography system with an expandable rack. The rack system gives built-in flexibility for the use of longer chromatographic columns, changes in system configuration, and increased automation. ÄKTAFPLC is controlled by UNICORN™, the control, evaluation, and reporting platform for ÄKTA™design systems, ensuring quick and simple communication between systems and users. UNICORN control system is designed for Windows 2000/XP and includes an option for network control of multiple systems. ÄKTAFPLC is compatible with all solvents commonly used in protein purification, including the high salt concentrations used in hydrophobic interaction chromatography.

Akta FPLC

The NanoDrop® ND-1000 UV-Vis Spectrophotometer 

The NanoDrop® ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses of 1 ul samples with remarkable reproducibility. The ND-1000 offers many benefits such as being designed for 1 ul samples, having a large dynamic range that measures 2-3700 ng/ul (dsDNA) on a single sample. No need to change out cuvettes or caps. High absorbance capability: 50 times that of traditional spectrophotometers, and no dilutions for most samples.

NanoDrop ND-1000

BIO-TEK - The ELx808™ Absorbance Microplate Reader 

ELx-808

BIO-TEK - Synergy™ HT Multi-Mode Microplate Reader 

The Synergy™ HT Multi-Mode Microplate Reader can be used for fluorescence, absorbance and luminescence measurements. Unlike many multi-mode microplate readers, the Synergy HT utilizes a unique dual-optics design which allows the investigator to have three different reading modes without compromise. It can read 6- to 384-well plate formats including PCR trays and Terasaki plates.

Synergy Microplate Reader

MJ Research - PTC 200 Thermal Cycler 

MJR PTC-200-The MJ Research PTC-200 is a dual 48-well block gradient thermocycler which allows two independent programs to be run at the same time. The PTC-200 also uses the Hot Bonnet to allow oil-free operation. Precise Peltier-effect heat pumps deliver the full temperature range of 0-100C electronically, in a compact instrument with a footprint that measures 24cm x 28cm x 27cm high. Speedy ramping rates of up to 3.0 sec in models that hold 0.2ml tubes or V-well plates

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