No Dividers Preview – Automation Facility

The Texas Institute for Drug and Diagnostics Development is currently bringing on-line an High Throughput Automation Facility. This facility has been established to foster drug discovery and development through the fully automated compound synthesis and (screening of commercial chemical libraries as well as in-house compounds. As a part of the Automation facility, the High Thfeeroughput Screening Facility (HTSF) is designed to be highly flexible in order to meet the needs of multiple investigators performing a variety of assays. In addition we aim to provide cost-effective and time-efficient access to high throughput screening technology across the UT-Campus as well as off-campus users. The facility staff assists assay development and miniaturization, will train users to use some of the instruments such as a microplate reader and liquid handling robot, and provide consultation for data analysis.

IMPORTANT – REQUEST FROM THE FACILITY:Users of the High throughput screening facility are urged to acknowledge any work performed by this Facility in their publications. Our service is a scientific support, not just a technical. Please send a copy of your publications to facility director, Eun Jeong Cho. We will compile the publications and may use as supporting material in the future for grant application such as Shared Instrumentation Grant application.

Wiki: http://wiki.ti3d.utexas.edu/

Screening Equipment

Perkin Elmer EnVision Microplate Reader

Application
General assay development utilizing various detection technology such as absorbance (UV-Vis), fluorescence intensity (FI), fluorescence polarization (FP), time- resolved fluorescence (TRF) and luminescence (Lum).

Specs

  • User-changeable label-specific optical mirror modules and filters
  • Superior detection sensitivity and high measurement speed (less than one min for 1536well plate with one flash/well)
  • 1-1536 microplate readin1-1536 microplate reading capability from bottom or top
  • Endpoint, kinetic, spectrum and well-area scanning read types
  • Temperature controlled (4-45°C)
  • Manual or automated serial plate reading via integrated plate stacker holding 50 plates
  • Filters

Manual
User Manual
Reference Manual

Web Site
EnVision

Top

Perkin Elmer MicroBeta TriLux Microplate Scintillation and Luminescence Counter*

Application
Specific for radioactive assay utilizing liquid scintillation or luminescence detection.

Specs

  • Samples in microplates, tubes or on filters
  • Sample capacitites of up to 16 or 32 plates
  • Updated model is fitted with an external loading platform for fully automatic loading by robot systems

* Located in PHR4.220B

.

Top

Jasco J-815 CD Spectrometer

Description
This machine enables high-throughput circular dichroism (CD) measurements from 96-microtube plates. CD spectra are very useful for studying the secondary structure of proteins, and their changes under different conditions.

Application
Specific assay development for optically active biological molecules (type of sugar or amino acid) and secondary structure of macromolecules to analyze for activity.
Specs

  • Circular dichroism (CD) and UV-Vis absorbance (standard)
  • Measurement from 163-900, 1100 nm optional
  • Single position Petier thermoelectric temperature control
  • Fluorescence accessory to measure fluorescence simultaneously
  • High throughput, temperature regulated automated sampling of up to 192 samples including thermal ramping and sample recovery capabilities

Website
J-815 CD Spectrometer

Top

Liquid Handling Equipment

Perkin Elmer Janus Workstation

Application
Laboratory automation workstation that performs versatile liquid handling tasks. Main task will be plate reformatting, large scale assay plating, and cherry picking.

Specs

  • Pipette tools consisted with 8tip varispan and 98/384 multi channel can transfer from 1 µ to 1 mL with high precisionthermo
  • Integrated stacker carousel can provide external supply and storage of microplates (50plates), deepwell plates, and P20 and P250 pipette tips
  • Faster throughput achieved with multichannel tools
  • Flexible with easy adaptation of any labwares or tips
  • Effective tracking of liquid levels is achieved with patented sonic liquid-sensing technology which accurately detects microliter volumes of both conductive and non-ionic liquids

Website
Janus Workstation

Top

Art Robbins Instruments Phoenix

Application
Laboratory automation workstation that performs low volume (lower than 1uL) liquid handling tasks. Main task will be library transferring and assay plate formation.
Specs

  • Simultaneous dispensing equipped with fixed 384 syringe head module and a single channel dispenser.
  • Fast liquid transferring speed (< 1 min) with a single channel head to 96 well plates.
  • Transfer liquids from 384-microwell source plates to 384/1536-microwell destination plates with very high accuracy from 100nL to 100uL.
  • 9 deck position are available for plates and washing stations.

Website
Phoenix

Top

BioTek Microflo Select

Application
Self contained, user programmable dispenser machine that dispenses a wide variety of liquids to micro-well plates.
Specs

  • Contains a peristaltic pump with eight individual tubes which transfer liquids from a supply bottle to various micro-well plates.
  • Able to dispense volumes from 1 to 9999ul using one of 3 cassettes.
  • Cassette sizes are 1ul, 5ul, and 10ul.
  • Plate stackers are able to hold up to 30 plates each (input and output).
  • Standard carrier is able to accommodate 6-, 12-, 48-, 96-, 384-, and 1536-well plates that comply with SBS microplate standards 4-2004.
  • Able to dispense a variety of solutions including buffered saline and reagent solutions.
  • Can store up to 99 dispense protocols.

Website
BioTek

Top

Synthesis Equipment

CEM Explorer 48 Automated Microrave Synthesizer

Description
Many reactions which require forcing conditions (high temperatures and reaction times) can benefit greatly from microwave irradiation. The Explorer 48 is easily programmed to automatically run up to 48 reactions in sequence, using 4- 10- or 35- mL reaction vials.

Web Site
Explorer 48

.

Top

.

CEM Liberty Automated Microwave Peptide Synthesizer

Description
The Liberty is an automated solid-phase peptide synthesizer which uses microwave irradiation to minimize reaction times and often give improved yields and purity over standard conditions. The system is capable of making up to 12 peptides a day at scales up to 5 mmol.

Web Site
Liberty

.

Top

.

Mettler-Toledo MiniBlock Peptide Synthesizer

Description
The MiniBlocks system is used for convenient parallel synthesis—up to 48 reactions at once in small test tubes (smallest size 4.5 mL). The reactions can be sealed under an inert atmosphere and heated or cooled under a large range of temperatures, either on a standard stir plate (with MiniBlock XT) or on a rotary shaker. Solid-phase synthesis is especially convenient with the MiniBlocks, as reaction solutions can be drained and filtered out the bottom of each reaction concurrently. Parallel work-up (purification) with e.g. solid-phase extraction (SPE) cartridges is also supported. The MiniBlock platform is great for the synthesis of chemical libraries, but it is also useful for reaction optimization, especially when used with the MiniMapper liquid handling robot.

Web Site
MiniBlock

Top

Mettler-Toledo MiniMapper liquid handler

Description
This liquid-handling robot can be easily programmed to add numerous solvents and reagent solutions to reaction vessels or microtiter plates. It can handle small quantities of corrosive reagents under an inert atmosphere. It is optimized for convenient use with the MiniBlock platform.

Web Site
MiniMapper

Top

Teledyne-Isco Companion 4x automated flash chromatography*

Description
This robot is set-up to purify up to 4 reactions in sequence, using normal-phase flash chromatography (typically with silica gel columns). It features a touch screen for rapid programming of conditions, dual pumps for forming solvent gradients, a dual wavelength UV detector, and automated fraction collector. It can purify crude reactions from milligram to multigram scale.
*Not located in the same room.

Web Site
Companion 4x

Top

Shimadzu analytical and preparative HPLC systems

Description
Two custom designed Shimadzu HPLC systems for high throughput analytical and preparative work. Both systems have cooled autosamplers with adjustable configuration for either two 96-well plates, or up to 70 standard HPLC vials, automated eight-solvent and six-column switching and PDA detectors. Both systems are also enabled with dual rack 3.5 ml x 70 tube fraction collection racks. The preparative system pumps can deliver up to 20 ml/minute and the preparative collection system can also be modified for large volume fraction collection.

Top

Genevac DD-4 centrifugal evaporator

Description
Conveniently removes both low- and high-boiling solvents (including DMSO) from test-tubes (e.g. generated by MiniBlock or prep. HPLC purifications) and microtiter plates.

.

Top

Thermo-Electron/Finnigan LTQ XL LC-MS*

Description
*Not located in the same room.

Web Site
LTQ XL

Top

Other Equipment

Thermo Scientific ALSP 3000 Plate Sealer

Application
Securely seal various plate types, which contain either chemical libraries dissolved in 100% DMSO solution or assay reagents for high throughput screening.
Specs

  • Uses five (5) standard and two (2) custom seals including clear sealing for both polystyrene and polypropylene plates.
  • The range of sealing plate height is 8 -46mm.
  • Operating temperature range is 15-40°C
  • Cycle time is between 6 to 12 seconds (per plate) depending on plate types, sealing time can be adjustable from 0.5s – 5s.
  • Sealing temperature can be controlled within the range of ambient to 200°C.

Web Site
Plate Sealer

Top

Zebra Z4M Plus Bar Code Printer

Application
Preparing bar coded lables for all screening processes.
Specs

  • Print Methods: Thermal Transfer.
  • Auto-calibration.
  • Resolution: 203dpi.
  • Print Speed: 10″ (254 mm) per Second.
  • Print length up to 157″ (3,988 mm).

Web Site
Z4M Printer

Top

Eppendorf Centrifuge 5810R with Swing-bucket Rotor

Application
Sample re-hydration and spin down.
Specs

  • Variable Speeds to 14,000 rpm.
  • Variable Time from 1-99 minutes.
  • Temperature Contraol from -4 – 40° C.
  • Print Speed: 10″ (254 mm) per Second.
  • Capability: 18 x 1.5 and 2.0 ml Tubes, 16 Microplates, 2 Culture Plates, 4 Deepwell Plates, or 2 Deepwell Assemblies (filter and catch-plate stacks)

Web Site
5810R Centrifuge

Top

VWR Signature Incubating Microplate Shaker

Application
Can be used for defrosting and mixing.
Specs

  • Can shake up to four microplates or two microtube racks with adjustable temperature
  • Speed range: 100 – 200 rpm
  • Temperature range: 5° C above ambient temperature to 65° C
  • Timer: 1 second – 160 hours

Web Site
VWR Incubator

Top

Data Analysis

TI-3D currently uses Collaborative Drug Discovery (CDD) for their Data Analysis needs. CDD’s secure web-based database system allows researchers to archive, mine, and collaborate with both other researchers in the lab and internationally. The application’s many features include compatibility with Excel, making it easy to store and download data to a public format; and the ability to restrict data or make it public, allowing researchers to both secure their data and share it with others.

CDD’s Website

CDD Application Tutorial
Batch Upload File for Tutorial

Top

Services and Fee Structure

  • NOTE: Fee schedule is now active, starting from April 1, 2009.
  • Fee Schedule is applied to on-campus users.
  • Off-campus users should contact the TI3D-HTSF Staff.
  • Pricing is highly dependent on the number of steps involved, the degree of multiplication, the length of preparation, and a number of other factors. Please contact TI3D-HTSF for project-specific pricing.

Please visit the wiki for more information.
Top

Chemical Library

Please refer to the wiki for our chemical library.
Top

Location

Mailing AddressThe University of Texas at Austin
1 University Station
Austin, TX 78712
Mail Code: A5300
.
Physical LocationThe University of Texas at Austin
Robert A. Welch Hall
Room 3.316
Map

Contact

OfficeDr. Eun Jeong Cho
1 University Station
Austin, TX 78712
Mail Code: A5300
Phone: (512) 471-5507
WEL 3.316
.
LabPhone: (512) 471-5485
WEL 3.316